Isolation and characterization of microbial population associated with industrial waste effluent and their antibiotic sensitive pattern
Isolation and characterization of microbial population associated with industrial waste effluent and their antibiotic sensitive pattern
Blog Article
Background: Both organic and inorganic chemicals are deposited in industrial effluents, which contain radioactive, metals, antibiotics, and carcinogenic substances.These effluents are directly or indirectly affecting the daily life of human.Through food chains, it migrates to human health and cased different diseases with different drug-resistant strains.Materials and Methods: Fifty-five industrial waste effluent samples were collected from waste effluent sites serially diluted and documented CFU from individual effluents and subcultured for isolated colony on nutrient agar plate, and then, grown bacteria were identified with culture morphology and biochemical tests.
Disc-diffusion method was used for antibiotic sensitivity pattern of isolated bacteria.Results: From 55 industrial waste samples, total 13 different types of bacterial strains were found from industrial waste effluent.Among all isolated bacteria, Pseudomonas sp., Staphylococcus epidermidis, Pseudomonas aeruginosa, gildan antique orange Bacillus sp.
, Acinetobacter sp., Staphylococcus aureus, Micrococcus sp., Citrobacter sp., Shigella sp.
, and Escherichia coli.Moreover, also 2 Aspergillus sp.and 2 unidentified fungus were revealed from this study.Antibiotic sensitivity pattern revealed that all the organisms show 100% resistant to amoxiclav, 71% resistant to ampicillin, 43% resistant to oxacillin antibiotics, 23% omni logic plus resistant to streptomycin, and 15% resistant to both gentamycin and tetracycline antibiotics.
Furthermore, we revealed 31% methicillin-resistant Staphylococcus aureus from industrial waste, whereas rest 69% revealed methicillin-susceptible Staphylococcus aureus strain.All the Gram-positive strains are shown highly resistant against beta-lactam group antibiotics.Conclusion: Our findings raise potential public health concerns for industrial waste effluent.Workers and individuals exposed to reclaimed wastewater.
Because of increasing use of reclaimed wastewater, further study is needed to evaluate the risk of exposure to antibiotic-resistant bacteria in treated wastewater.